Abstract: To study the effects of Eucommia ulmoides leaf powder on body composition, muscle amino acid composition, physiological indicators of yellow river carp, the Eucommia ulmoides leaf powder were added into the diet at the concentrations of 0, 2%, 4%, 6% and 8%. 225 individuals with an average weight of (505.13±1.37) g were selected and fed for 55 days. The experimental data showed that: (1) Fresh drosal muscle lipid content and fresh body moisture content decreased significant while fresh drosal muscle protein and fresh body contents of crud protein, crud lipid and crud ash were significant improved with the increasing of Eucommia ulmoides leaf powder (P<0.05). (2) 16 kinds of amino acids were detected in the muscle of yellow river carp. The fresh content of Thr, Ser and His were significantly affected by the adding of Eucommia ulmoides leaf powder. The ratio of total acidic amino acids to total amino acids was significantly higher while the ratio of total pharmacodynamic amino acids to total amino acids was significantly lower in the 2% treatment compared with others (P<0.05). (3) The activities of GSH-PX, ACP, AKP and MDA content in plasma and liver significantly increased to the highest level and then decreased with the increased addition of Eucommia ulmoides leaf powder. SOD activity of Plasma and liver increased significant according with the increasing Eucommia ulmoides leaf powder (P<0.05). In plasma, the activities of GSH-PX and content of MDA were all significantly higher in 6% treatment. AKP activity showed the highest in 4% treatment while ACP activity showed the highest in 8% treatment, respectively. SOD activity increased significantly to the control level at 8% treatment. In liver, GSH-PX activity and MDA content were the highest in 4% treatment (P<0.05). AKP activity and ACP activity were the highest in 6% treatment and 4% treatment respectively (P<0.05). SOD activity showed the highest at 8% treatment (P<0.05). In conclusion, according to the fish drosal muscle and body composition, muscle amino acid, antioxidant and non-specific immune indexes in plasma and liver, the recommended level of diet Eucommia ulmoides in yellow river carp should be at 4%—6%.